Endocrine Abstracts

The cytosolic calcium concentration is regulated by calcium-processing proteins such as transient receptor potential cation channel subfamily V member 5 (TRPV5), TRPV6, sodium-calcium exchanger 1 (NCX1) and plasma membrane Ca2+ ATPase 1 (PMCA1). Those calcium-processing proteins are important for physiological functions in the brain. The effects of steroid hormones on calcium-processing protein expressions in the brains are undescribed. Thus, the effects of steroid ...

Endocrine-disrupting chemicals (EDCs) have similar structures with steroids hormones, which can interfere with hormone synthesis and normal physiological functions of male and female reproductive organs. Sex steroid hormones influence calcium signaling of the cardiac muscle in early embryo development. Progesterone (P4) has been reported to reduce blood pressure. To confirm the effect of P4, octyl-phenol (OP) and bisphenol A (BPA) on early differentiation of mouse embryonic st...

Ki-67 can be solely detected within the cell nucleus, whereas in mitosis, most of the Ki-67 proteins are located on the chromosome surface. Ki-67 is present during all phases of the cell cycle (G1, S, G2, and M), but BrdU is only present in the S phase. This study examined whether it is possible to establish a developmental neurotoxicity test in human neural progenitor cells using Ki-67 instead of BrdU (5-bromo-2’-deoxyuridine). In the present study, Ki-67-expressed ReNce...

Developmental toxicity tests have been made by embryonic stem cell tests at the European Centre for the Validation of Alternative Methods or by embryonic body test in our laboratory. However, no neuronal-specific developmental toxicity test has been made yet. Therefore, this study was carried out using a 46C cell line, mouse embryonic stem cells with an endogenous Sox1-GFP reporter, to exploit the developmental neurotoxicity test. The expression of Sox1, a marker for neural pr...